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KMID : 1138520050080030005
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Journal of Pharmacopuncture
2005 Volume.8 No. 3 p.5 ~ p.9
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Fluorescent Method for Observing Intravascular Bonghan Duct
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Lee Byung-Cheon
Baik Ku-Youn
Johng Hyeon-Min
Sung Baeck-Kyoung
Soh Kyung-Soon
Kang Dae-In
Soh Kwang-Sup
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Abstract
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Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining revealed the distinctive pattern of nuclei distribution that clearly discerned the threadlike structure from fibrin, capillary, small venule, arteriole, or lymph vessel. Physiological function of the intra-vascular thread in connection with acupuncture is discussed. Especially, this threadlike duct can be a circulation path for herb-liquid flow, which may provide the scientific mechanism for therapeutic effect of herbal acupuncture.
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KEYWORD
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Bonghan duct, Intra-vascular thread, Confocal microscope, Acridine-orange, Herbal acupuncture
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